Protein-Protein Interactions

Tools and Approaches

Slides:
https://cytoscape.github.io/cytoscape-tutorials/presentations/ppi-tools1-2017-mpi.html

John "Scooter" Morris
Alex Pico

Max Planck Institute for Biology of Ageing
27 November 2017

Today's Agenda

TimeTopic
10:00 - 11:00Introductions - Why are protein-protein interactions important?
11:00 - 12:30Protein-protein interaction networks - I
13:30 - 15:00Protein-protein interaction networks - II
  • Network approaches to visualizing and analyzing protein-protein interactions
  • Introduction to Cytoscape - I
15:00 - 15:30Break
15:30 - 17:00Introduction to Cytoscape - II
  • Hands-on
17:00 - 18:00Workflows
Introductions PPI networks - I PPI networks - II Introduction to Cytoscape Workflows

Introductions

John "Scooter" Morris
  • 2010 - Current
    • Adjunct Assistant Professor, Pharmaceutical Chemistry, UCSF
  • 2004 - Current
    • Executive Director, NIH Resource for Biocomputing, VIsualization, and Informatics
  • 1985 - 2004
    • Principal Systems Architect: Genentech, Inc.
  • 2006 - Current
    • Cytoscape core team
  • Author or co-author of several Cytoscape apps:
    • structureViz2, clusterMaker2, chemViz2, stringApp, CyAnimator

Introductions

Alex Pico, Gladstone Inst.
  • Associate Director, Bioinformatics
  • Executive Director NRNB
  • Cytoscape team since 2006
  • Author of half a dozen Cytoscape apps
  • Founder of the WikiPathways repository

Introductions

What about you?

  • Computational Biologist
  • Structural Biologist
  • Biologist
  • Chemist
  • Bioinformatician
  • Computer Scientist
  • Data Scientist
  • Other?

What is this course about?

    Protein-protein interactions
  • Some background
  • Some techniques
  • Two tools:
  • Cytoscape - network visualization and analysis
  • UCSF Chimera - structural visualization and analysis
  • Why those tools?
  • It's what our lab works on ☺

Why?

    Why are protein-protein interactions important?
  • You tell me...

Why?

    Break into groups of 5-7 people
  • Each person:
    • Name
    • Introduce your image
    • Please keep it short
  • Choose one image to present to everyone
Introductions PPI networks - I PPI networks - II Introduction to Cytoscape Workflows
Introductions PPI networks - I PPI networks - II Introduction to Cytoscape Workflows
Introductions PPI networks - I PPI networks - II Introduction to Cytoscape Workflows
Introductions PPI networks - I PPI networks - II Introduction to Cytoscape Workflows

Hands-on Tutorials

Questions

  • Specific workflows you are interested in?
  • Any specific "How to" questions?

Thank You!

Here are additional resources you may find useful: